"Hyperspectral Imaging
For Bio-Medical, Biological and
Materials Science"
PARISSR Hyperspectral Imaging works in:
Fluorescence, Absorption, Reflection, Emission
and Dark-Field Scatter
The PARISS system is a microscope accessory
( PARISS: "Prism and Reflector Imaging Spectroscopy System")
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| Spectral emission profiles of Alexa 555, 569, and 594. Emission spectra of each fluorophore strongly overlaps the others. |
Tissue section stained with Alexa 555, 569 and 594; all excited simultaneously at 488 nm. The distribution of each fluorophore is clearly segmented**. |
| Key Hyperspectral Imaging Functions |
- Spectral range 365 to 920 nm in a single shot
- Spectral "unmixing" with auto-spectral libraries
- Characterize CFP/YFP FRET with over 300 wavelength data points!
- Conduct pure spectral analysis in absorption, % reflection, %transmission on micro-samples
- Generate spectral histograms for statistical analysis of regions of interest or entire fields
- Radiometric calibration to compensate for variations in camera sensitivity (QE), as a function of wavelength
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| Key Hyperspectral Imaging Applications |
- Fluorescence Segmentation: delineate up to 15 fluorophores simultaneously
- Nanoparticle, Nanowire and Nanorod characterization: Characterize individual or fields of nanoparticles for size and shape in dark-field scatter
- FRET and multiplexed fluorophore imaging
- Advanced segmentation and analysis of bright-field histological samples
- OLED defect characterization
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To play the PARISS Hyperspectral Imaging
"animated" presentation Click Here |
* "Alexa Fluor(R)" and "Molecular Probes(TM)" are trademarks of Invitrogen/Molecular Probes
** Taken from "Abstract_FRET_FLIM_Spectral Imaging2" Click here for the pdf
PARISSR, MIDLR and Sylph(tm) are trademarks of LightForm, Inc.
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c 1990-2010 LightForm, Inc., all rights reserved
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